2 edition of induction and isolation of herbicide resistant mutants of Mimulus cardinalis found in the catalog.
induction and isolation of herbicide resistant mutants of Mimulus cardinalis
Roberta Crowell Barbalace
Written in English
|Statement||by Roberta Crowell Barbalace|
|The Physical Object|
|Pagination||39 leaves :|
|Number of Pages||39|
Isolation of Arabidopsis Mutants Resistant to Disease Arabidopsis ecotype Columbia (Col-0) is susceptible to P. s. tomato DC Disease symptoms develop on rosette leaves 4 to 5 days after inoculation and appear as gray le-sions surrounded by chlorosis (Whalen et al., ). To iden-tify Arabidopsis mutants with reduced susceptibility, weCited by: Herbicide-resistant mutants from tobacco cell cultures. Chaleff RS, Ray TB. Several mutants resistant to the herbicides chlorsulfuron and sulfometuron methyl were isolated form cultured cells of Nicotiana tabacum. Resistance was inherited as a single dominant or semidominant mutation in all by:
Mutation induction, together with mutation detection a key element of mutation breeding, has been an important tool for plant breeders for more than 70 years to increase the genetic diversity of plants and derive new mutant lines with improved characteristics. Isolation and Characterization of Arabidopsis Mutants Defective in the Induction of Ethylene Biosynthesis by Cytokinin John P. Vogel,1 Peter Schuerman, Keith Woeste, Ingrid Brandstatter and Joseph J. Kieber Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois at Chicago, Chicago, Illinois Cited by:
CULLIN1. antiauxin-resistant mutants (aars) aar, aar4, and aar5 were also resistant to 2,4-dichlorophenoxyacetic acid as shown by a root growth assay. Positional cloning of aar revealed that the AAR3 gene encodes a protein with a domain of unknownCited by: We used grain amaranth (Amaranthus hypochondriacus L.) and resistance to acetolactate synthase (ALS)-inhibiting herbicides as a model system to discover spontaneous herbicide-resistant mutants. After screening million plants, however, we detected no spontaneous resistant genotypes, indicating the probability of finding a spontaneous ALS Cited by: 2.
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Similarly, mutants resistant to an antibiotic or a toxic chemical can be isolated by plating the microbial population with the antibiotic or the chemical. Replica-Plating Technique: Replica-plating technique is often used to isolate nutritional mutants (auxotrophs) as well as various other type of mutants, e.g., antibiotic resistant mutants.
ing each of the herbicide-resistant mutations coupled with the auxotrophic mutation arg7 were crossed to herbicide- resistant mutants carrying the complementing arg2 muta- tions, and after days of growth in continuous light on 2% minimal medium agar plates (-arginine), prototrophic diploid colonies were by: Molecular and Biochemical Parasitology, 10 (I) 89 Elsevier MBP INDUCTION AND SELECTION OF DRUG RESISTANT MUTANTS OF PLASMODIUM FALCIPAR UM JOSEPH INSELBURG Department of Microbiology, Dartmouth Medical School Hanover, NHU.S.A.
(Received 17 March ; accepted 17 June ) Conditions for mutagenizing Cited by: AUXOTROPHIC MUTANTS IN MAMMALIAN CELLS 31 TABLE II Composition of Saline G Concentration Chemicals (gm/liter) Preparation NaCl Dissolved in 1 liter (final volume) KC1 of triple-distilled water, MgSO„-7H20 sterilized and stored at 4°C CaClH20 NajHPCV 7H20 KH2P04 Glucose Phenol Red efficiency as in Cited by: 2.
They provide useful material for cell biological studies. Some of the mutants like the pathotoxin resistant, amino-acid-analogue resistant (3) and herbicide resistant (4) Author: N.
Gupta, S. Bhaskaran. isolation of mutants: ISOLATION OF MUTANTS Mutation occurring in microorganism can be detected and efficiently isolated from the parent organism of other mutants. While studying we must be aware of wild type characters of an organism,so the mutants can easily detected.
PDF | The effect of various mutagenic treatments on vegetative and floral characters of different cultivars of tuberose (Polianthes tuberosa L) was | Find, read and cite all the research you. The isolation of analogue-resistant mutants has already been discussed in the field of primary metabolism, where the rationale was that a mutant resistant to the inhibitory effects of an analogue, which mimics the control characteristics of the natural metabolite, may overproduce the natural approach has been adopted, or may be adopted, in a number of guises in the field of.
Summary. Selection in vitro can be a very powerful tool because very large numbers of cells can be utilized. The mutants that are selected can have value both in basic studies and for agricultural and other commercial purposes, such as drug by: 1.
Sulfonylurea resistant mutants of Arabidopsis thaliana, Nicotiana tabacum, Saccharomyces cerevisiae and Saccharomyces typhimurium were isolated and most of them showed acetolactate synthase activity insensitive to the herbicides and the activity of resistant enzyme cosegregated with cellular resistance in genetic crosses [5,28,35].
D Chamovitz, G Sandmann, J HirschbergCited by: This method helps in isolation of both nutritional mutants as well as antibiotic resistant mutants.
It involves replicating the original plate having sensitive cells into two. you are here->home->Biotechnology and Biomedical Engineering->Microbiology Virtual Lab II->Isolation and Identification of Auxotrophic and Drug Resistant Mutants. Isolation and Identification of Auxotrophic and Drug Resistant Mutants.
Theory. Procedure. Self Evaluation The importance of replica plating method in the isolation of. Ji Hoon Ahn, Joonki Kim, Seong Jeon Yoo, So Yeon Yoo, Hyungmin Roh, Jun-Hyuk Choi, Mi Suk Choi, Kyung Sook Chung, Eun Ju Han, Sung Myun Hong, Sung Hye Jung, Hyo Jin Kang, Bo Kyung Kim, Mi Duk Kim, Youn Kyung Kim, Yun Hee Kim, Hanna Lee, Soo Hyun Park, Jee Hoon Yang, Jung Won Yang, Dong-Hun Yoo, Seung Kwan Yoo, Jong Seob Lee, Isolation of Mutants that Cited by: Isolation and characterization of novel mutants of Arabidopsis thaliana defective in flower development MASAKO K.
KOMAKI1, KIYOTAKA OKADA1, EISHO N1SHINO2 and YOSHIRO SHIMURA1 1 Division of Cellular Communication, National Institute for Basic Biology, OkazakiJapan 2Biological Laboratory, College of Arts and Sciences, Chiba University, Cited by: Isolation of mutant 1.
Made by:Shveta Arya Isolation of Mutant 2. Positive Selection Positive selection entails growing the culture on a medium that will allow for the growth of only the mutant colonies.
If, for example, we want to find mutants that resistant to penicillin, we grow the culture on a medium that contains penicillin. Only those colonies that are resistant to penicillin. Isolation and Characterization of Mutants in Plant Cell Culture.
Annual Review of Plant Physiology Vol. (Volume publication date June ) Comparison of breeding methods used in modern agriculture. Cross breeding: improving a trait (e.g., disease resistance) through crossing an elite recipient line with a donor line and selecti Cited by: Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection.
The selection of drug resistant mutants in vitro can be readily accomplished by maintaining the virus in a. Isolation of metabolic analogue-resistant mutants -Azotobacter chroococcum strain A I 03 was used to obtain spontaneous mutants resistant to Msx, Mal and Mac.
This strain showed high nitrogenase activity under cultural conditions and moderate ammonia excretion in Burk's medium15• The mtmmumCited by: 8. Aluminum (Al) toxicity represents a major constraint for crop production on acid soils. Rice is a high Al-resistant plant species among small-grain cereals, but its molecular mechanisms of Al resistance are not fully understood.
We adopted a forward genetic screen strategy to uncover the Al-resistance mechanisms in rice. In this study, we screened an ethylmethylsulfone (EMS)-mutagenized Cited by: 4. Two mutants of Arabidopsis thaliana that are resistant to growth inhibition by indoleacetic acid (IAA)-phenylalanine have been isolated.
Both mutants were 2- to 3-fold more resistant than wild type to inhibition by IAA-phenylalanine, IAA-alanine, and IAA-glycine in root growth assays.
The mutant icr1 (but not icr2) also shows some resistance to by: We used grain amaranth (Amaranthus hypochondriacus L.) and resistance to acetolactate synthase (ALS)-inhibiting herbicides as a model system to discover spontaneous herbicide-resistant mutants.
After screening million plants, however, we detected no spontaneous resistant genotypes, indicating the probability of finding a spontaneous ALS-resistant mutant in a given Cited by: 2.Recently Viewed. The Journal of Physical Chemistry A.
Mechanistic Photochemistry of Methylhydroxycinnamate Chromophore and Its One-Water Complexes: Insights from MS-CASPT2 StudyCited by: